抗体工程组


(一)本组简介

本研究小组由罗文新副教授担任导师,在读博士生6名,在读硕士生4名,技术员3名,本科生若干。本小组的主要研究方向为抗体药物的研发,近年来在抗体药物研发领域进行了较为深入的研究,围绕基于治疗抗体药物研发的关键技术环节,初步建立了一个从全人抗体、猴源抗体和纳米抗体筛选、抗体分子改造到抗体表达生产工艺和成药性评估的抗体药物开发技术平台,从而推进基础研究中获得的创新性治疗抗体向产业化转化。此外,建立了双特异抗体、抗体药物偶联物、清道夫抗体等新型抗体构建平台和CAR-T细胞治疗平台,在慢乙肝感染、手足口病、肝癌、乳腺癌等病毒感染性疾病和肿瘤治疗方面取得了良好的效果。

目前正在开展的项目包括HBV二代治疗性抗体研究、HBV治疗及诊断用纳米抗体研究、肠道病毒双特异抗体研究、靶向PD-1的双特异抗体用于治疗三阴性乳腺癌的研究、CAR-T用于治疗脑胶质瘤的研究、募集T细胞的双特异抗体用于肝癌治疗的研究等。

本小组已完成多个国家、省、市项目,目前承担2个国家基金、1个传染病重大专项,发表SCI论文20余篇,申请中国发明专利18项,国际发明专利4项,获得授权14项,完成HBV治疗性抗体的成药性评估及临床前分子筛选,已推进到产业化转化阶段。

(二)导师

罗文新

(三) 近5年来发表的部分文章

1.  Liu X, Liu C, Zheng Z, Chen S, Pang X, Xiang X, Tang J, Ren E, Chen Y, You M, Wang X, Chen X, Luo W*, Liu G*, Xia N*. Vesicular Antibodies: A Bioactive Multifunctional Combination Platform for Targeted Therapeutic Delivery and Cancer Immunotherapy. Adv Mater. 2019 Mar 7:e1808294. doi: 10.1002/adma.201808294

2.  Shen C, Zhang M, Chen Y, Zhang L, Wang G, Chen J, Chen S, Li Z, Wei F, Chen J, Yang K, Guo S, Wang Y, Zheng Q, Yu H, Luo W, Zhang J, Chen H, Chen Y, Xia N. An IgM antibody targeting the receptor binding site of influenza B blocks viral infection with great breadth and potency. Theranostics. 2019 Jan 1;9(1):210-231

3.  Deng M, Gui X, Kim J, Xie L, Chen W, Li Z, He L, Chen Y, Chen H, Luo W, Lu Z, Xie J, Churchill H, Xu Y, Zhou Z, Wu G, Yu C, John S, Hirayasu K, Nguyen N, Liu X, Huang F, Li L, Deng H, Tang H, Sadek AH, Zhang L, Huang T, Zou Y, Chen B, Zhu H, Arase H, Xia N, Jiang Y, Collins R, You MJ, Homsi J, Unni N, Lewis C, Chen GQ, Fu YX, Liao XC, An Z, Zheng J, Zhang N, Zhang CC. LILRB4 signalling in leukaemia cells mediates T cell suppression and tumour infiltration. Nature. 2018 Oct;562(7728):605-609.

4.  Zhou B, Xu L, Zhu R, Tang J, Wu Y, Su R, Yin Z, Liu D, Jiang Y, Wen C, You M, Dai L, Lin Y, Chen Y, Yang H, An Z, Fan C*, Cheng T*, Luo W*, Xia N. A bispecific broadly neutralizing antibody against enterovirus 71 and coxsackievirus A16 with therapeutic potential. Antiviral Res. 2018 Nov 9. pii: S0166-3542(18)30463-7

5.  Wang X, An Z, Luo W, Xia N, Zhao Q. Molecular and functional analysis of monoclonal antibodies in support of biologics development. Protein Cell. 2018 Jan;9(1):74-85.

6.  Zhang C, Huang X, Chen S, Li Y, Li Y, Wang X, Tang J, Xia L, Lin Z, Luo W, Li T, Li S, Zhang J, Xia N, Zhao Q. Epitope clustering analysis for vaccine-induced human antibodies in relationship to a panel of murine monoclonal antibodies against HPV16 viral capsid. Vaccine. 2018 Oct 29;36(45):6761-6771

7.  You M, Yang Y, Zhong C, Chen F, Wang X, Jia T, Chen Y, Zhou B, Mi Q, Zhao Q, An Z, Luo W*, Xia N. Efficient mAb production in CHO cells with optimized signal peptide, codon, and UTR. Appl Microbiol Biotechnol. 2018 May 8. doi: 10.1007/s00253-018-8986-5.

8.  Yang Y, You M, Chen F, Jia T, Chen Y, Zhou B, Mi Q, An Z, Luo W*, Xia N*. Efficient Development of a Stable Cell Pool for Antibody Production Using a Single Plasmid. J Biochem. 2018,163(5):391-398

9.  Kang C, Xia L, Chen Y, Zhang T, Wang Y, Zhou B, You M, Yuan Q, Tzeng CM, An Z, Luo W*, Xia N. A novel therapeutic anti-HBV antibody with increased binding to human FcRn improves in vivo PK in mice and monkeys. Protein Cell. 2018, 9(1):130-134.

10.  Wang X, An Z, Luo W, Xia N, Zhao Q*. Molecular and functional analysis of monoclonal antibodies in support of biologics development. Protein Cell. 2018 Jan;9(1):74-85.

11.  Xia L, Su R, An Z, Fu TM, Luo W*.  Human Cytomegalovirus vaccine development: Immune responses to look into vaccine strategy. Human Vaccines & Immunotherapeutics..2018,14(2):292-303.

12.  Xia L, Tang A, Meng W, Freed DC, He L, Wang D, Li F, Li L, Xiong W, Gui X, Schultz RD, Chen H, He X, Swoyer R, Ha S, Liu Y, Morris CD, Zhou Y, Wang IM, Zhao Q, Luo W, Xia N, Espeseth AS, Hazuda DJ, Rupp RE, Barrett AD, Zhang N, Zhu J, Fu TM, An Z. Active evolution of memory B-cells specific to viral gH/gL/pUL128/130/131 pentameric complex in healthy subjects with silent human cytomegalovirus infection. Oncotarget. 2017, 8(43):73654-73669

13.  Shen C, Chen J, Li R, Zhang M, Wang G, Stegalkina S, Zhang L, Chen J, Cao J, Bi X, Anderson SF, Alefantis T, Zhang M, Cai X, Yang K, Zheng Q, Fang M, Yu H, Luo W, Zheng Z, Yuan Q, Zhang J, Wai-Kuo Shih J, Kleanthous H, Chen H, Chen Y, Xia N. A multimechanistic antibody targeting the receptor binding site potently cross-protects against influenza B viruses. Sci Transl Med. 2017 Oct 18;9(412). pii: eaam5752. doi: 10.1126/scitranslmed.aam5752.

14.  Xia L, Xian Y, Wang D, Chen Y, Huang X, Bi X, Yu H, Fu Z, Liu X, Li S, An Z, Luo W*, Zhao Q, Xia N*. A human monoclonal antibody against HPV16 recognizes an immunodominant and neutralizing epitope partially overlapping with that of H16.V5. Sci Rep. 2016, 6:19042. doi: 10.1038/srep19042.

15.  Zhang TY, Yuan Q, Zhao JH, Zhang YL, Yuan LZ, Lan Y, Lo YC, Sun CP, Wu CR, Zhang JF, Zhang Y, Cao JL, Guo XR, Liu X, Mo XB, Luo WX, Cheng T, Chen YX, Tao MH, Shih JW, Zhao QJ, Zhang J, Chen PJ, Yuan YA, Xia NS. Prolonged suppression of HBV in mice by a novel antibody that targets a unique epitope on hepatitis B surface antigen. Gut, 2015.doi:10.1136/gutjnl-2014-308964.

16.  Xia L, Zhang J, Cui C, Bi X, Xiong J, Yu H, An Z, Luo W*, Xia N*. In vitro affinity maturation and characterization of anti-P24 antibody for HIV diagnostic assay. J Biochem. 2015, 158(6):531-538.

17.  Tao Jiang, Daiping Guo, Qian Wang, Xin Wu, Zhao Li, Zhenhua Zheng, Boyuan Yin, Lin Xia, Jixian Tang, Wenxin Luo, Ningshao Xia, Yunbao Jiang. Developing a Genetically Encoded Green Fluorescent Protein Mutant for Sensitive Light-up Fluorescent Sensing and Cellular Imaging of Hg(II). Anal Chim Acta. 2015,876: 77-82

18.  You M, Xin L, Yang Y, Zhang X, Chen Y, Yu H, Li S, Zhang J, An Z, Luo W*, Xia N*. Investigation of a special neutralizing epitope of HEV E2s. Protein Cell. 2014 Dec;5(12):950-953.

(四)本组优势

毕业生去向包括养生堂、药明康德、赛诺菲等知名企业,从事抗体表达和抗体人源化改造等研发工作,以及在生物、药学、医学相关高校或研究机构任职等。

优秀学生有可能获得推荐,前往美国德州大学西南医学中心等合作单位学习。

(五)招生要求

生物学、药学、医学相关专业。

只要你有一颗乐于探索的心,勇于进取的科研精神,积极向上的生活观,对抗体工程感兴趣,我们欢迎你的加入。


上一条:临床研究组 下一条:POCT研究组

国家传染病诊断试剂与疫苗工程技术研究中心(厦门大学)  地址:中国福建省厦门市翔安区翔安南路厦门大学翔安校区
电话:0592-2183111 传真:0592-2181258  邮编:361102
ICP备案号:D200327