疫苗与结构生物学组

(一)本组简介

人员构成:教师3人、高级工程师1人、博士后1人、学生33人、技术员7人,共45人。

研究方向:基因工程疫苗研究与开发、重要传染病病毒免疫表位的结构和功能研究、基于结构的分子模拟和分子设计、生物大分子药物质量分析与制剂研究、新型疫苗佐剂研发。

技术平台:基因工程疫苗研发平台、基于CRISPR-Cas9技术的大肠杆菌基因组改造、杆状病毒表达系统、哺乳动物细胞表达系统、X-ray衍射晶体结构解析平台、Cryo-EM结构解析平台、分子动力学计算平台、二代测序平台、生物大分子药物质量分析平台、佐剂研发平台。

研究内容:

1. HPV系列:基于结构的人乳头病毒(HPV)型交叉疫苗研发、HPV抗体中和机制研究、HPV主要衣壳蛋白L1与次要衣壳蛋白L2相互作用研究、L2蛋白结构研究、HPV致癌蛋白的功能机制研究。

2. HEV系列:戊型肝炎病毒(HEV)广谱中和抗体和型特异抗体表位及机制研究;基于HEV结构设计的通用的颗粒化疫苗研究。

3. VZV系列:基于水痘-带状疱疹病毒表面糖蛋白gE的重组疫苗研发与结构解析研究。

4. 流感、肠道病毒等的结构研究:解析流感的血凝素蛋白(HA)和肠道病毒多种衣壳形式与中和抗体的复合物结构,研究抗体介导的病毒中和作用机制。

5. 疫苗质量控制:重组疫苗相关质量分析方法的建立(生物物理、生物化学、免疫化学方法)、重组疫苗体外相对效力分析、生物药物后期转化。

6. 新型佐剂:新型佐剂筛选及成药性研究、佐剂及抗原-佐剂间的相互作用分析、佐剂免疫调节机制探索。

(二)导师

李少伟、赵勤俭、俞海

(三)正在开展的项目

1HEV/HPV多价联合疫苗研究与产品开发

2. 肠道病毒抗原性差异的结构基础

3. 基因工程疫苗研发和生产用的大肠杆菌表达系统的基因组功能改造和应用

4. 基于结构的流感HA关键表位研究

5. 带状疱疹疫苗研究及相关蛋白结构解析

6. 基于矿化双膦酸微纳米颗粒的新型佐剂筛选及免疫作用机制研究

7. 新冠重组疫苗体外相对效力实验及制剂稳定性研究

8. 重组疫苗/蛋白(HPV九价苗、乙肝治疗性蛋白)质量分析方法的建立

 

(四)近5年来发表的部分文章

  1. Longfa Xu#, Qingbing Zheng#, Rui Zhu#, Zhichao Yin#, Hai Yu#, Yu Lin, Yuanyuan Wu, Maozhou He, Yang Huang, Yichao Jiang, Hui Sun, Zhenghui Zha, Hongwei Yang, Qiongzi Huang, Dongqing Zhang, Zhenqin Chen, Xiangzhong Ye, Jinle Han, Lisheng Yang, Che Liu, Yuqiong Que, Mujin Fang, Ying Gu, Jun Zhang, Wenxin Luo, Z. Hong Zhou*, Shaowei Li*, Tong Cheng*, Ningshao Xia*. Cryo-EM Structures Reveal Basis of Receptor-Initiated Coxsackievirus Uncoating with Implications for Therapeutic Antibody Development. Cell Host Microbe, 2021, S1931-3128(21)00001-9. IF= 15.923

  2. Maozhou He#, Xin Chi#, Zhenghui Zha#, Yunbing Li, Jie Chen, Yang Huang, Shiwen Huang, Miao Yu, Zhiping Wang, Shuo Song, Xinlin Liu, Shuangping Wei, Zekai Li, Tingting Li, Ying-Bin Wang, Hai Yu, Qinjian Zhao, Jun Zhang, Qing-Bing Zheng*, Ying Gu*, Shaowei Li*, and Ning-Shao Xia; Structural basis for the shared neutralization mechanism of three classes of human papillomavirus type 58 antibodies with disparate modes of binding. Journal of Virology, 2021, 01587-20.

  3. He, Maozhou#; Xu, Longfa#; Zheng, Qingbing#; Zhu, Rui#; Yin, Zhichao#; Zha, Zhenghui; Lin, Yu; Yang, Lisheng; Huang, Yang; Ye, Xiangzhong; Li, Shuxuan; Hou, Wangheng; Wu, Yangtao; Han, Jinle; Liu, Dongxiao; Li, Zekai; Chen, Zhenqin; Yu, Hai; Que, Yuqiong; Wang, Yingbin; Yan, Xiaodong; Zhang, Jun; Gu, Ying; Zhou, Z. Hong*; Cheng, Tong*; Li, Shaowei*; Xia, Ningshao*; Identification of Antibodies with Non-overlapping Neutralization Sites that Target Coxsackievirus A16, Cell Host & Microbe, 2020, 27(2): 249-261.

  4. Daning Wang#; Xinlin Liu#; Minxi Wei#;Ciying Qian; Shuo Song; Jie Chen; Zhiping Wang; Qin Xu; Yurou Yang; Maozhou He; Xin Chi; Shiwen Huang; Tingting Li; Zhibo Kong; Qingbing Zheng; Hai Yu; Yingbin Wang; Qinjian Zhao; Jun Zhang; Ningshao Xia*;Ying Gu*& Shaowei Li*;Rational design of a multi-valent human papillomavirus vaccine by capsomere-hybrid co-assembly of virus-like particles., Nature Communications, 2020, 11(1):2841.

  5. Wei Wang#;Qingbing Zheng#; Dequan Pan#; Hai Yu#; Wenkun Fu, Jian Liu, Maozhou He, Rui Zhu, Yuze Cai, Yang Huang, Zhenghui Zha, Zhenqin Chen, Xiangzhong Ye, Jinle Han, Yuqiong Que, Ting Wu, Jun Zhang, Shaowei Li*;Hua Zhu*;Z. Hong Zhou*;Tong Cheng*and Ningshao Xia*;Near-atomic cryo-electron microscopy structures of varicella-zoster virus capsids. Nature Microbiology, 2020,5(12) :1542-1552.

  6. Lizhi Zhou#; Hai Yu#; Kaihang Wang; Tingting Chen; Yue Ma; Yang Huang; Jiajia Li; Liqin Liu; Yuqian Li; Zhibo Kong; Qingbing Zheng; Yingbin Wang; Ying Gu; Ningshao Xia and Shaowei Li*; Genome re-sequencing and reannotation of the Escherichia coli ER2566 strain and transcriptome sequencing under overexpression conditions, BMC Genomics, 2020, 21(1):407.

  7. Tingting Li#; Qingbing Zheng#* ;Hai Yu#; Dinghui Wu#; Wenhui Xue#; Hualong Xiong#; Xiaofen Huang; Meifeng Nie; Mingxi Yue; Rui Rong; Sibo Zhang; Yuyun Zhang; Yangtao Wu; Shaojuan Wang; Zhenghui Zha; Tingting Chen; Tingting Deng; Yingbin Wang; Tianying Zhang; Yixin Chen; Quan Yuan*; Qinjian Zhao; Jun Zhang; Ying Gu*; Shaowei Li *and Ningshao Xia*; SARS-CoV-2 spike produced in insect cells elicits high neutralization titres in non-human primates, Emerging Microbes & Infections, 2020; 9(1): 2076–2090

  8. Weijin Huang  1 , Maozhou He  2 , Tingting Ning #  3 , Jianhui Nie  1 , Feng Zhang  4 , Qingbing Zheng  2 , Rui Zhang  5 , Ying Xu  1 , Ying Gu  2 , Shaowei Li  2 , Youchun Wang;Structural characterization of a neutralizing mAb H16.001 that is qualified to a common potency assay for various HPV16 vaccines. NPJ Vaccines,2020,5:89.

  9. Ciying Qian#; Xinlin Liu#; Qin Xu; Zhiping Wang; Jie Chen; Tingting Li; Qingbing Zheng ; Hai Yu ;Ying Gu*; Shaowei Li * and Ningshao Xia; Recent Progress on the Versatility of Virus-Like Particles.Vaccines,2020, 20;8(1):139.

  10. Qingbing Zheng#; Rui Zhu#; Longfa Xu#; Maozhou He#; Xiaodong Yan#; Dongxiao Liu; Zhichao Yin; Yangtao Wu; Yongchao Li; Lisheng Yang; Wangheng Hou; Shuxuan Li; Zizhen Li; Zhenqin Chen; Zhihai Li; Hai Yu; Ying Gu; Jun Zhang; Timothy S Baker; Z Hong Zhou; Barney S Graham*; Tong Cheng*; Shaowei Li*; Ningshao Xia*; Atomic structures of enterovirus D68 in complex with two monoclonal antibodies define distinct mechanisms of viral neutralization , Nature Microbiology, 2019, 4(1): 124-133.

  11. Zheng, Qingbing#; Jiang, Jie#; He, Maozhou#; Zheng, Zizheng#; Yu, Hai; Li, Tingting; Xue, Wenhui; Tang, Zimin; Ying, Dong; Li, Zekai; Song, Shuo; Liu, Xinlin; Wang, Kaihang; Zhang, Zhiqing; Wang, Daning; Wang, Yingbin; Yan, Xiaodong; Zhao, Qinjian; Zhang, Jun; Gu, Ying*; Li, Shaowei*; Xia, Ningshao*; Viral neutralization by antibody-imposed physical disruption, Proceedings of the National Academy of Sciences, 2019, 116(52): 26933-26940.

  12. Chen, Tingting#; Wang, Kaihang#; Chi, Xin; Zhou, Lizhi; Li, Jiajia; Liu, Liqin; Zheng, Qingbing; Wang, Yingbin; Yu, Hai; Gu, Ying; Zhang, Jun; Li, Shaowei*; Xia, Ningshao; Construction of a bacterial surface display system based on outer membrane protein F , Microbial Cell Factories, 2019, 18: 0-70.

  13. Xinlin Liu#; Jie Chen#; Zhiping Wang#; Daning Wang; Maozhou He; Ciying Qian; Shuo Song; Xin Chi; Zhibo Kong; Qingbing Zheng; Yingbin Wang; Hai Yu; Qinjian Zhao; Jun Zhang; Shaowei Li*; Ying Gu*; Ningshao Xia; Neutralization sites of human papillomavirus-6 relate to virus attachment and entry phase in viral infection, Emerging Microbes & Infections, 2019, 8(1): 1721-1733.

  14. Kai hang Wang#; Lizhi Zhou#; Xiao Zhang#; Cuiling Song; Tingting Chen; Jiajia Li; Minghua Zheng; Yingbin Wang; Qingbing Zheng; Zizheng Zheng; Hai Yu; Ting Wu; Ying Gu; Jun Zhang; Qinjian Zhao*; Shaowei Li*; Ningshao Xia; Hepatitis E vaccine candidate harboring a non-particulate immunogen of E2 fused with CRM197 fragment A, Antiviral Research, 2019, 2019(164): 154-161.

  15. Li Zhihai#; Song Shuo#; He Maozhou#; Wang Daning; Shi Jingjie; Liu Xinlin; Li Yunbing; Chi Xin; Wei Shuangping; Yang Yurou; Wang Zhiping; Li Jinjin; Qian Huilian; Yu Hai; Zheng Qingbing; Yan Xiaodong; Zhao Qinjian; Zhang Jun; Gu Ying*; Li Shaowei*; Xia Ningshao*; Rational design of a triple-type human papillomavirus vaccine by compromising viral-type specificity., Nature Communications, 2018, 9(1): 0-5360.

  16. Zhu Rui#; Xu Longfa#; Zheng Qingbing#; Cui Yanxiang#; Li Shaowei#; He Maozhou; Yin Zhichao; Liu Dongxiao; Li Shuxuan; Li Zizhen; Chen Zhenqin; Yu Hai; Que Yuqiong; Liu Che; Kong Zhibo; Zhang Jun; Baker Timothy S; Yan Xiaodong*; Hong Zhou Z*; Cheng Tong*; Xia Ningshao*; Discovery and structural characterization of a therapeutic antibody against coxsackievirus A10. Science Advances, 2018, 4(9): 0-eaat7459.

  17. Zhang,Zhiqing#; He, Maozhou#; Bai,shimeng#; Zhang,Feng; Jiang,Jie; Zheng,Qingbing; Gao,shuangquan; Yan,xiaodong; Li,Shaowei*; Gu,Ying*; Xia,Ningshao; T = 4 Icosahedral HIV-1 Capsid As an Immunogenic Vector for HIV-1 V3 Loop Epitope Display, Viruses, 2018, 10(12).

  18. Wei Minxi#; Wang Daning#; Li Zhihai#; Song Shuo; Kong Xianglin; Mo Xiaobing; Yang Yurou; He Maozhou; Li Zhongyi; Huang Bo; Lin Zhijie; Pan Huirong; Zheng Qingbing; Yu Hai; Gu Ying; Zhang Jun; Li Shaowei*; Xia Ningshao*; N-terminal truncations on L1 proteins of human papillomaviruses promote their soluble expression in Escherichia coli and self-assembly in vitro., Emerg Microbes Infect, 2018, 7(1): 0-160.

  19. Lin Qingshan#; Li Tingting#; Chen Yixin#; Lau Siu Ying; Wei Minxi; Zhang Yuyun; Zhang Zhenyong; Yao Qiaobin; Li Jinjin; Li Zhihai; Wang Daning; Zheng Qingbing; Yu Hai; Gu Ying; Zhang Jun; Chen Honglin; Li Shaowei*; Xia Ningshao*; Structural Basis for the Broad, Antibody-Mediated Neutralization of H5N1 Influenza Virus , Journal of Virology, 2018, 92(17): e00547-18.

  20. Li, Zhihai#; Wang, Daning#; Gu, Ying#; Song, Shuo; He, Maozhou; Shi, Jingjie; Liu, Xinlin; Wei, Shuangping; Li, Jinjin; Yu, Hai; Zheng, Qingbing; Yan, Xiaodong; Baker, Timothy S.; Zhang, Jun; McLellan, Jason S.*; Li, Shaowei*; Xia, Ningshao*; Crystal Structures of Two Immune Complexes Identify Determinants for Viral Infectivity and Type-Specific Neutralization of Human Papillomavirus, MBio, 2017, 8(5).

  21. Xu, Longfa#; Zheng, Qingbing#; Li, Shaowei#; He, Maozhou; Wu, Yangtao; Li, Yongchao; Zhu, Rui; Yu, Hai; Hong, Qiyang; Jiang, Jie; Li, Zizhen; Li, Shuxuan; Zhao, Huan; Yang, Lisheng; Hou, Wangheng; Wang, Wei; Ye, Xiangzhong; Zhang, Jun; Baker, Timothy S.; Cheng, Tong*; Zhou, Z. Hong; Yan, Xiaodong*; Xia, Ningshao*; Atomic structures of Coxsackievirus A6 and its complex with a neutralizing antibody, Nature communications, 2017, 11;8(1):505.

  22. Li, Zhihai#; Yan, Xiaodong#; Yu, Hai#; Wang, Daning; Song, Shuo; Li, Yunbing; He, Maozhou; Hong, Qiyang; Zheng, Qingbing; Zhao, Qinjian; Gu, Ying; Zhang, Jun; Janssen, Mandy E W; Cardone, Giovanni; Olson, Norman H; Baker, Timothy S*; Li, Shaowei*; Xia, Ningshao; The C-Terminal Arm of the Human Papillomavirus Major Capsid Protein Is Immunogenic and Involved in Virus-Host Interaction., Structure, 2016, 24(6): 874-885.

上一条:溶瘤病毒研究组 下一条:临床研究组

国家传染病诊断试剂与疫苗工程技术研究中心(厦门大学)  地址:中国福建省厦门市翔安区翔安南路厦门大学翔安校区
电话:0592-2183111 传真:0592-2181258  邮编:361102
ICP备案号:D200327