感染模型与疫苗组


(一)本组简介

本研究小组负责人为程通副教授,成员包括博士后5名,在读博士生5名,在读研究生12名,技术员4名,是一支富有创新精神和的凝聚力的学术团队。主要研究方向包括重要新发/突发病毒的基础病毒学和新型疫苗研究,病毒感染致病机制研究,新型溶瘤病毒和新靶标,新型人源化动物模型和皮肤功效因子等研究。近5年内,本组承担了国家传染病重大专项、国家重大新药创制专项课题、国家863计划、国家自然科学基金等十余项课题研究,在Nature MicrobiologyScience AdvancesNature CommunicationsGutTheranosticsEMIAntiviral ResVirologyVaccine等权威学术期刊发表研究论文50余篇,已获得了9项中国发明专利和4项美国、欧盟、日本发明专利授权。

本组近年来取得了多项重要研究成果:(1)与美国Rutgers大学合作,应用病毒功能基因组学与反向遗传学技术研制出新型水痘病毒活疫苗(VZV-7D),是世界首个皮肤与神经双减毒水痘活疫苗,已获得国家一类新药临床试验批件,目前正在开展I期临床试验研究;(2)在儿童手足口病(HFMD)病原体肠道病毒的优势中和表位发现和新型多价疫苗研究上取得突破,应用分子病毒学与结构生物学技术首次获得了重要肠道病毒CVA6、CVA10和EVD68的病毒颗粒及其与优势中和抗体复合物的高分辨率三维结构,发现了优势中和表位和潜在治疗靶标,相关成果分别发表于Nature和Science杂志子刊;(3)与浙江大学合作,应用干细胞、人鼠嵌合模型与病毒学技术首次建立了基于人骨髓间充质干细胞移植的人肝细胞和人免疫细胞双重嵌合的人源化小鼠模型,是首个可高度模拟人类乙肝病毒自然感染和乙肝肝硬化发病过程的动物模型,相关成果发表权威期刊Gut杂志,受到广泛关注;(4)在探索新型溶瘤病毒用于胰腺癌、肝癌、肺癌等癌症治疗研究、肿瘤新靶标研究、新型植物病毒载体和新型皮肤功效改善因子研究等也取得重要进展。

(二) 导师

程通

(三)正在开展的项目

1、新型人源化动物模型研究

2、水痘-带状疱疹病毒疫苗与病毒学研究  

3、肠道病毒广谱疫苗与病毒学研究  

4、新型溶瘤病毒与肿瘤治疗靶标研究

5、新型植物病毒载体研究

6、新型皮肤功效改善因子研究

(四) 近5年来发表的部分研究论文

[1]  Yuan L#, Jiang J#, Liu X#, Zhang Y, Zhang L, Xin J, Wu K, Li X, Cao J, Guo X, Shi D, Li J, Jiang L, Sun S, Wang T, Hou W, Zhang T, Zhu H, Zhang J, Yuan Q, Cheng T*, Li J*, Xia N*. HBV infection-induced liver cirrhosis development in dual-humanized mice with human bone mesenchymal stem cell transplantation. Gut. 2019, 0:1–13.

[2]  Zheng Q#, Zhu R#, Xu L#, He M#, Yan X#, Liu D, Yin Z, Wu Y, Li Y, Yang L, Hou W, Li S, Li Z, Chen Z, Li Z, Yu H, Gu Y, Zhang J, Baker TS, Zhou ZH, Graham BS*, Cheng T*, Li S*, Xia N*. Atomic Structures of Enterovirus D68 in Complex with Two Therapeutic Antibodies Define Distinct Mechanisms of Viral Neutralization. Nature Microbiology. 2019, 4(1):124-133

[3]  Zhu R#, Xu L#, Zheng Q#, Cui Y#, Li S#, He M, Yin Z, Liu D, Li S, Li Z, Chen Z, Yu H, Que Y, Liu C, Kong Z, Zhang J, Baker TS, Yan X*, Hong Zhou Z*, Cheng T*, Xia N*.Discovery and structural characterization of a therapeutic antibody against coxsackievirus A10. Science Advances. 2018, 4(9):eaat7459

[4]  Xu L, Zheng Q, Li S, He M, Wu Y, Li Y, Zhu R, Yu H, Hong Q, Jiang J, Li Z, Li S, Zhao H, Yang L, Hou W, Wang W, Ye X, Zhang J, Baker TS, Cheng T*, Zhou ZH, Yan X*, Xia N*. Atomic structures of Coxsackievirus A6 and its complex with a neutralizing antibody. Nature Communications. 2017, 8(1):505.

[5]  Yuan L, Liu X, Zhang L, Zhang Y, Chen Y, Li X, Wu K, Cao J, Hou W, Que Y, Zhang J, Zhu H, Yuan Q*, Tang Q*, Cheng T*, Xia N. Optimized HepaRG is a suitable cell source to generate human liver chimeric mouse model for chronic hepatitis B virus infection. Emerg Microbes Infect. 2018, 7(1):144

[6]  Yuan L, Liu X, Zhang L, Li X, Zhang Y, Wu K, Cao J, Hou W, Zhang J, Zhu H, Yuan Q*, Tang Q*, Cheng T*, Xia N. A Chimeric Humanized Mouse Model by Engrafting the Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cell for the Chronic Hepatitis B Virus Infection. Frontiers in Microbiology. 2018, 9:908

[7]  Li S, Armstrong N, Zhao H, Hou W, Liu J, Chen C, Wan J, Wang W, Zhong C, Liu C, Zhu H, Xia N, Cheng T*, Tang Q*. Zika Virus Fatally Infects Wild Type Neonatal Mice and Replicates in Central Nervous System. Viruses. 2018, 10(1):pii:E49

[8]  Wang W, Yang L, Huang X, Fu W, Pan D, Cai L, Ye J, Liu J, Xia N, Cheng T*, Zhu H*. Outer nuclear membrane fusion of adjacent nuclei in varicella-zoster virus-induced syncytia. Virology. 2017, 512:34-38.

[9]  Wang W, Fu W, Pan D, Cai L, Ye J, Liu J, Liu C, Que Y, Xia N, Zhu H*, Cheng T*. Varicella-zoster virus ORF7 interacts with ORF53 and plays a role in its trans-Golgi network localization. Virologica Sinica. 2017, 32(5):387-95

[10]  Wang W, Pan D, Fu W, Cai L, Ye J, Liu J, Liu C, Huang X, Lin Y, Xia N, Cheng T*, Zhu H*. A SCID mouse-human lung xenograft model of varicella-zoster virus infection. Antiviral Research. 2017, 146:45-53.

[11]   Li S, Zhao H, Yang L, Hou W, Xu L, Wu Y, Wang W, Chen C, Wan J, Ye X, Liang Z, Mao Q*, Cheng T*, Xia N. A neonatal mouse model of coxsackievirus A10 infection for anti-viral evaluation. Antiviral Research. 2017, 144:247-55

[12]  Wu Y, Zhu R, Xu L, Li Y, Li S, Yu H, Li S, Zhu H, Cheng T*, Xia N. A novel combined vaccine based on monochimeric VLP co-displaying multiple conserved epitopes against Enterovirus 71 and Varicella-Zoster Virus. Vaccine. 2017, 35(20):2728-35.

[13]  Yang L, Liu Y, Li S, Zhao H, Lin Q, Yu H, Huang X, Zheng Q, Cheng T*, Xia N. A novel inactivated enterovirus 71 vaccine can elicit cross-protective immunity against coxsackievirus A16 in mice. Vaccine. 2016, 34:5938-45

[14]  Yang L, Mao Q, Li S, Gao F, Zhao H, Liu Y, Wan J, Ye X, Xia N, Cheng T*, Liang Z*. A neonatal mouse model for the evaluation of antibodies and vaccines against coxsackievirus A6. Antiviral Research. 2016, 134:50-57

[15]  Wang W, Wang X, Yang L, Fu W, Pan D, Liu J, Ye J, Zhao Q, Zhu H, Cheng T*, Xia N. Modulation of host CD59 expression by varicella-zoster virus in human xenografts in vivo. Virology. 2016, 491:96-105.

[16]  Zhu R, Liu J, Chen C, Ye X, Xu L, Wang W, Zhao Q, Zhu H, Cheng T*, Xia N. A highly conserved epitope-vaccine candidate against varicella-zoster virus induces neutralizing antibodies in mice. Vaccine. 2016. 34:1589-96.

[17]  Ye X, Yang L, Jia J, Han J, Li S, Liu Y, Xu L, Zhao H, Chen Y, Li Y, Cheng T*, Xia N. Development of sandwich ELISAs that can distinguish different types of coxsackievirus A16 viral particles. Appl Microbiol Biotechnol. 2016, 100(6):2809-15

[18]  Yang L, Li S, Liu Y, Hou W, Lin Q, Zhao H, Xu L, He D, Ye X, Zhu H, Cheng T*, Xia N. Construction and characterization of an infectious clone of coxsackievirus A6 that showed high virulence in neonatal mice. Virus Res. 2015, 210:165-168

[19]  Liu J, Zhu R, Ye X, Yang L, Wang Y, Huang Y, Wu J, Wang W, Ye J, Li Y, Zhao Q, Zhu H, Cheng T*, Xia N. A monoclonal antibody-based VZV Glycoprotein E Quantitative Assay and Its Application on Antigen Quantitation in VZV Vaccine. Appl Microbiol Biotechnol, 2015, 99(11):4845-53

[20]  Hou W, Yang L, Li S, Yu H, Xu L, He D, Chen M, He S, Ye X, Que Y, Shih JW, Cheng T*, Xia N*. Construction and characterization of an infectious cDNA clone of Echovirus 25. Virus Res. 2015, 205:41-44

[21]  Yang L, He D, Tang M, Li Z, Liu C, Xu L, Chen Y, Du H, Zhao Q, Zhang J, Cheng T*, Xia N. Development of an enzyme-linked immunosorbent spot assay to measure serum-neutralizing antibodies against coxsackievirus B3. Clin Vaccine Immunol. 2014, 21(3):312-320

[22]  Xu L, He D, Li Z, Zheng J, Yang L, Yu M, Yu H, Chen Y, Que Y, Shih JW, Liu G, Zhang J, Zhao Q, Cheng T*, Xia N. Protection against lethal Enterovirus 71 challenge in mice by a recombinant vaccine candidate containing a broadly cross-neutralizing epitope within the VP2 EF loop. Theranostics. 2014, 4(5):498-513. (cover paper)

(五) 招生条件

1. 热爱科学研究,开朗乐观。

2. 具有较强的实验动手能力,责任心和协作精神。


下一条:感染机制组

国家传染病诊断试剂与疫苗工程技术研究中心(厦门大学)  地址:中国福建省厦门市翔安区翔安南路厦门大学翔安校区
电话:0592-2183111 传真:0592-2181258  邮编:361102
ICP备案号:D200327