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顾颖

颖,博士  GU Ying, Ph.D.

副教授,硕士生导师Associate Professor

话:+86-592-2187880

真:+86-592-2181258

E-mailguying@xmu.edu.cn

 

 

简历 (Education and Work experience)

1996年,厦门大学生物学专业,学士学位;

2006年,厦门大学生化与分子生物学专业/细胞生物学专业,博士学位;

2006年,厦门大学生命科学学院助理教授、硕士生导师;

2013年,厦门大学生命科学学院副教授;

B.S. Biochemistry, Xiamen University, 2000

Ph.D. Biochemistry & Molecular Biology/Cell Biology, Xiamen University, 2006

Assistant Professor, School of Life Sciences, Xiamen University, 2006

 

Associate Professor, School of Life Sciences, Xiamen University, 2013

 

研究领域 (Research Area)

 

  • 抗病毒的单抗筛选:利用细胞模型和重组抗原等进行抗病毒的单抗筛选,如HIV中和单抗、识别免疫优势表位单抗等的筛选。

  • 基于表位的抗原研究:利用基因工程重组技术表达获得含有功能表位的抗原或颗粒化抗原。

  • 功能性表位研究:应用生物传感器、分析超离、微量热等方法研究抗原-抗体的相互作用,尝试解析免疫复合物的空间结构,鉴定功能性表位的特征。

  • Generation of monoclonal antibodies against different viral targets : based on functional screening by virus-infection cell model, recombinant antigen reactivity and et. al, focusing on HIV neutralizing monoclonal antibodes, esp. those with broad neutralizing activity

  • Identification and characterization of key epitopes on viral antigens: Design and preparation of recombinant antigens harbouring functional epitopes

  • Functional characterization of epitopes: epitope mapping, structural analysis and function interpretation by various methods including SPR-based assay, analytical ultracentrifugation, microcalorimetry, and structural determination of antibody-antigen complexes

代表性论文(Selected Publications)(* Corresponding author)

  1. Gu Y, Cao F, Wang L, Hou W, Zhang J, Hew CL, Li S*, Yuan YA*, Xia N*. Structure of a Novel Shoulder-to-Shoulder p24 Dimer in Complex with the Broad-Spectrum Antibody A10F9 and Its Implication in Capsid Assembly. PLoS One. 2013 Apr 19;8(4):e61314.

  2. Yang C, Pan H, Wei M, Zhang X, Wang N, Gu Y, Du H, Zhang J,Li S*, Xia N*.Hepatitis E virus capsid protein assembles in 4M urea in the presence of salts.Protein Sci. 2013 Mar;22(3):314-26.

  3. Gu Y, Hou W, Xu C, Li S, Shih JW, Xia N*. The enhancement of RNAi against HIV in vitro and in vivo using H-2K(k) protein as a sorting method. J Virol Methods. 2012 Jun;182(1-2):9-17.

  4. Shen L1, Gu Y 1, Sun L, Yang Y, Wang F, Li Y, Bi S*. Development of a hepatitis delta virus antibody assay for study of the prevalence of HDV among individuals infected with hepatitis B virus in China. J Med Virol. 2012 Mar;84(3):445-9. (co-1st)

  5. Tang X, Yang C, Gu Y, Song C, Zhang X, Wang Y, Zhang J, Hew CL,Li S*, Xia N*, Sivaraman J*. Structural basis for the neutralization and genotype specificity of hepatitis E virus. ProcNatl Acad Sci U S A. 2011 Jun 21;108(25):10266-71.

  6. Yu H,Li S*, Yang C, Wei M, Song C, Zheng Z, Gu Y, Du H, Zhang J, Xia N*. Homology model and potential virus-capsid binding site of a putative HEV receptor Grp78. J Mol Model.2011 May;17(5):987-95.

  7. Zheng Z, Luo W*, Song H, Chen Y, Xiong J, Chen Y, Gu Y, Chen H, Shih JW, Zhang J, Xia N*. Antibody reactivity of conformational peptide mimics of a conserved H5N1 neutralization site in different fusion proteins. Arch Virol. 2010;155(1):19-26.

  8. Li S, Tang X, Seetharaman J, Yang C, Gu Y, Zhang J, Du H, Shih JW, Hew CL, Sivaraman J*, Xia N*. Dimerization of Hepatitis E Virus Capsid Protein E2s Domain is Essential for Virus-Host Interaction.PLoS Pathog. 2009 Aug;5(8):e1000537.

  9. Xu CY, Gu Y*, Hou WH, Que YQ, Gao SG, Cheng T, Xia NS*. Tetracysteine as a Reporter for Gene Therapy. Biomed Environ Sci. 2009 Dec;22(6):496-501.

  10. Li S, Zhang J, He Z, Gu Y, Liu R, Lin J, Chen Y, Ng M, Xia N*. Mutational analysis of essential interactions involved in the assembly of hepatitis E virus capsid.J Biol Chem. 2005 Feb 4;280(5):3400-6.

  11. Yang HJ, Chen M, Cheng T, He SZ, Li SW, Guan BQ, Zhu ZH, Gu Y, Zhang J, Xia NS.Expression and immunoactivity of chimeric particulate antigens of receptor binding site-core antigen of hepatitis B virus. World J Gastroenterol. 2005 Jan 28;11(4):492-7.

  12. Zhang J, Gu Y, Ge SX, Li SW, He ZQ, Huang GY, Zhuang H, Ng MH, Xia NS. Analysis of hepatitis E virus neutralization sites using monoclonal antibodies directed against a virus capsid protein. Vaccine. 2005 Apr 22;23(22):2881-92.

  13. Gu Y, Zhang J, Wang YB, Li SW, Yang HJ, Luo WX, Xia NS*. Selection of a peptide mimicking neutralization epitope of hepatitis E virus with phage peptide display technology. World J Gastroenterol. 2004 Jun 1;10 (11):1583-8.

  14. Zhang J, Ge SX, Huang GY, Li SW, He ZQ, Wang YB, Zheng YJ, Gu Y, Ng MH, Xia NS. Evaluation of antibody-based and nucleic acid-based assays for diagnosis of hepatitis E virus infection in a rhesus monkey model. J Med Virol. 2003 Dec;71(4):518-26.

 

荣誉、奖励及参加学术团体的情况(Honors and award)

 

主持3项国家自然科学基金项目、1项国家科技重大专项子课题,1项福建省科技重大项目,并作为主要参与人参加多项国家级、省部级重大重点科研项目;获得中国高校科技进步一等奖(2001),国家技术发明二等奖(2010),中国专利金奖(2012),厦门市科学技术进步一等奖(2010),厦门大学第四届青年教师教学技能比赛一等奖(2008),厦门大学第三届青年教师教学技能比赛二等奖(2007。申请发明专利11项,国际发明专利8项,获得授权4项;为主参与研制戊肝疫苗、宫颈癌疫苗、艾滋诊断试剂等新药。

  • WIPO-SIPO Award for Chinese Outstanding Patented Invention (2012)

  • State Technological Invention Award (2010)

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